Replication of Syngrapha falcifera Multiple-Nuclear Polyhedrosis Virus-D in Different Insect Cells
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چکیده
منابع مشابه
The Role of Cytochrome c on Apoptosis Induced by Anagrapha falcifera Multiple Nuclear Polyhedrosis Virus in Insect Spodoptera litura Cells
There are conflicting reports on the role of cytochrome c during insect apoptosis. Our previous studies have showed that cytochrome c released from the mitochondria was an early event by western blot analysis and caspase-3 activation was closely related to cytochrome c release during apoptosis induced by baculovirus in Spodoptera litura cells (Sl-1 cell line). In the present study, alteration i...
متن کاملIdentification of seven putative origins of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus DNA replication.
Seven putative origins of DNA replication (oris) were identified and located on the genome of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus (AcMNPV), when an improved infection-dependent replication assay was used. A threefold higher yield of amplified plasmid was achieved when an m.o.i. of 1 was used (instead of 25), and another twofold increase was obtained when the ...
متن کاملCharacterization of nuclear polyhedrosis virus DNAs.
The nuclear polyhedrosis virus DNAs characterized and compared in this study consist of the singly-enveloped nucleocapsids (SNPV) of Trichoplusia ni and the bundles of nucleocapsids common to a single envelope (MNPV) from Spodoptera frugiperda and Rachiplusia ou. The SNPV and MNPV DNAs are very similar in hydrodynamic properties and molecular weights. In addition, the NPV DNAs are similar in si...
متن کاملVirus envelope acquisition of nuclear polyhedrosis virus in vitro.
There have been several reports concerned with the replication and morphogenesis of insect baculoviruses during the past decade [1--7]. While there is general agreement as to the assembly of the virus on the basis of electron microscopic studies, there are still questions regarding the details of the replicative mechanisms, such as the acquisition of the virus envelope. Three possible ways have...
متن کاملBaculovirus Replication: Stimulation of Thymidine Kinase and DNA Polymerase Activities in Spodopterafrugiperda Cells Infected with Trichoplusia ni Nuclear Polyhedrosis Virus
Baculoviruses are large DNA-containing viruses which replicate in the nucleus of invertebrate cells. The viruses contain supercoiled DNA of tool. wt. about 8 x l 0 7 (Bud & Kelly, 1977) and the multiply enveloped nuclear polyhedrosis virus of Trichoplusia ni used in this study has a genome size of 8.3 x 107 (Bud & Kelly, 1980). Little is known about the replication of baculoviruses in molecular...
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ژورنال
عنوان ژورنال: Journal of Applied Sciences
سال: 2008
ISSN: 1812-5654
DOI: 10.3923/jas.2008.1756.1760